PXD022684

PXD022684 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of FPA interactors
Description	Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat receptor genes (NLRs) are found in plant genomes and provide disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, controlling their functional expression and impacting immunity. Using long-read nanopore direct RNA sequencing we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in immunity.
HostingRepository	PRIDE
AnnounceDate	2020-12-16
AnnouncementXML	Submission_2020-12-16_04:00:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Parker
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 01:24:22	ID requested
⏵ 1	2020-12-16 04:00:01	announced

Dataset with its publication pending

submitter keyword: Arabidopsis,FPA,RNA processing,transcription

Gordon Simpson
contact affiliation	The University of Dundee
contact email	g.g.simpson@dundee.ac.uk
lab head
Matthew Parker
contact affiliation	The University of Dundee
contact email	mtparker@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022684

PRIDE project URI

• PRIDE
  1. PXD022684
     1. Label: PRIDE project
     2. Name: Proteomic analysis of FPA interactors