PXD022670

PXD022670 is an original dataset announced via ProteomeXchange.

Title	β-Adrenergic regulation of metabolism in macrophages
Description	Macrophages have important roles in the immune system including clearing pathogens and wound healing. Metabolic phenotypes have been associated with functional phenotypes, where pro-inflammatory macrophages have an increased rate of glycolysis and anti-inflammatory macrophages primarily use oxidative phosphorylation. β-adrenoceptor (βAR) signalling in macrophages has been implicated in disease states such as cancer, atherosclerosis and rheumatoid arthritis. The impact of β-adrenoceptor signalling on macrophage metabolism has not been defined. Here we expand on defining the phenotype of macrophages treated with isoprenaline and describe the impact that βAR signalling has on the metabolome and proteome. We found that βAR signalling alters proteins involved in cytoskeletal rearrangement and redox control of the cell. We showed that βAR signalling in macrophages shifts glucose metabolism from glycolysis towards the tricarboxylic acid cycle and pentose phosphate pathways. We also show that βAR signalling perturbs purine metabolism by accumulating adenylate pools. Taken together these results indicate that βAR signalling shifts metabolism to support redox perturbations and upregulate proteins involved in cytoskeletal changes that may impact migration and phagocytosis processes.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:32:01.496.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amanda Peterson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-23 09:09:37	ID requested
⏵ 1	2021-11-03 05:32:02	announced

Peterson AL, Siddiqui G, Sloan EK, Creek DJ, -Adrenoceptor regulation of metabolism in U937 derived macrophages. Mol Omics, 17(4):583-595(2021) [pubmed]

submitter keyword: human, macrophage, LC-MSMS

Darren John Creek
contact affiliation	Drug Delivery, Disposition and Dynamics Theme, Monash Institute of Pharmaceutical Science, Monash University, Parkville, Victoria, Australia
contact email	darren.creek@monash.edu
lab head
Amanda Peterson
contact affiliation	Monash University
contact email	amanda.peterson@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022670
     1. Label: PRIDE project
     2. Name: β-Adrenergic regulation of metabolism in macrophages