PXD022668

PXD022668 is an original dataset announced via ProteomeXchange.

Title	Distinct extracellular-matrix remodeling events precede symptoms of inflammation
Description	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment. Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix. In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity. Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects. We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes. Remarkably, whether the inflammation is chronic or acute, its matrix signature converges at pre-symptomatic states. We suggest that the existence of a pre-symptomatic extracellular-matrix is general and relevant to a wide range of diseases.
HostingRepository	PRIDE
AnnounceDate	2020-12-02
AnnouncementXML	Submission_2020-12-01_23:04:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Idan Adir
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-23 08:04:45	ID requested
⏵ 1	2020-12-01 23:04:45	announced

Dataset with its publication pending

submitter keyword: Extracellular matrix, proteomics, electron microscopy, proteolysis, inflammation, colitis

Ulrich auf dem Keller
contact affiliation	Technical University of Denmark (lab head)
contact email	uadk@dtu.dk
lab head
Idan Adir
contact affiliation	PhD student at Irit Sagi's lab in the Weizmann institure
contact email	idan.adir@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022668

PRIDE project URI

• PRIDE
  1. PXD022668
     1. Label: PRIDE project
     2. Name: Distinct extracellular-matrix remodeling events precede symptoms of inflammation