PXD022665

PXD022665 is an original dataset announced via ProteomeXchange.

Title	Comprehensive quantitative proteome analysis of Aedes aegypti to interrogate Wolbachia pipientis and Zika virus interference phenomenon
Description	Zika virus is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of Zika virus, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remains to be elucidated. In this study, we apply quantitative isobaric mass spectrometry-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the Ae. aegypti. We show that Wolbachia regulates proteins involved in ROS production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in the mosquitoes was determined by mass spectrometry and corroborates the idea that Wolbachia helps to block ZIKV infections in Ae. aegypti. The present study offers a rich resource of data to help elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in Ae. aegypti.
HostingRepository	PRIDE
AnnounceDate	2021-02-26
AnnouncementXML	Submission_2021-02-25_22:40:19.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luis Felipe Ramos
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-23 08:04:29	ID requested
⏵ 1	2021-02-25 22:40:20	announced

Dataset with its publication pending

submitter keyword: Aedes aegypti, head, ZIKV, Wolbachia

Magno Junqueira
contact affiliation	Proteomics Unit, Biochemistry Department, Chemistry Institute, Federal University of Rio de Janeiro.
contact email	magnojunqueira@iq.ufrj.br
lab head
Luis Felipe Ramos
contact affiliation	Federal University of Rio de Janeiro
contact email	luisfelipecostaramos@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022665

PRIDE project URI

• PRIDE
  1. PXD022665
     1. Label: PRIDE project
     2. Name: Comprehensive quantitative proteome analysis of Aedes aegypti to interrogate Wolbachia pipientis and Zika virus interference phenomenon