PXD022664

PXD022664 is an original dataset announced via ProteomeXchange.

Title	A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
Description	Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI/SNF family of chromatin remodelling complexes in an NCAPH2-independent manner. This cross-reactivity with an abundant chromatin-associated factor is likely to affect the interpretation of protein and chromatin immunoprecipitation experiments that make use of this antibody probe.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-14_00:23:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimi Wills
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-23 07:48:09	ID requested
⏵ 1	2020-12-14 00:23:46	announced

Dataset with its publication pending

submitter keyword: antibody, cross-react, condensin, ncaph2, swi/snf

Andrew J Wood
contact affiliation	MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Edinburgh, EH4 2XU, UK
contact email	andrew.wood@igmm.ed.ac.uk
lab head
Jimi Wills
contact affiliation	University of Edinburgh
contact email	jimi.wills@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022664

PRIDE project URI

• PRIDE
  1. PXD022664
     1. Label: PRIDE project
     2. Name: A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component