PXD022660

PXD022660 is an original dataset announced via ProteomeXchange.

Title	The ER protein Ema19 facilitates the degradation of non-imported mitochondrial precursor proteins
Description	For the biogenesis of mitochondria, hundreds of proteins need to be targeted from the cytosol into the various compartments of this organelle. The intramitochondrial targeting routes these proteins take to reach their respective location in the organelle are well understood. However, the early targeting processes, from cytosolic ribosomes to the membrane of the organelle, are still largely unknown. In this study, we present evidence that an integral membrane protein of the endoplasmic reticulum (ER), Ema19, plays a role in this process. Mutants lacking Ema19 show an increased stability of mitochondrial precursor proteins, indicating that Ema19 promotes the proteolytic degradation of non-productive precursors. The deletion of Ema19 improves the growth of respiration-deficient cells, suggesting that Ema19-mediated degradation can compete with productive protein import into mitochondria. Ema19 is the yeast representative of a conserved protein family. The human Ema19 homolog is known as sigma-2 receptor or TMEM97. Though its molecular function is not known, previous studies suggested a role of the sigma-2 receptor as a quality control factor in the ER, compatible with our observations about Ema19. More globally our data provide an additional demonstration of the important role of the ER in mitochondrial protein targeting.
HostingRepository	PRIDE
AnnounceDate	2021-03-24
AnnouncementXML	Submission_2021-03-24_07:58:44.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-23 07:47:50	ID requested
⏵ 1	2021-03-24 07:58:45	announced

Laborenz J, Bykov YS, Kn, ö, ringer K, R, ä, schle M, Filker S, Prescianotto-Baschong C, Spang A, Tatsuta T, Langer T, Storchov, á Z, Schuldiner M, Herrmann JM, The ER protein Ema19 facilitates the degradation of nonimported mitochondrial precursor proteins. Mol Biol Cell, 32(8):664-674(2021) [pubmed]

submitter keyword: LC-MS/MS, Mitochondrial Protein Import, Mitochondria , AE-MS

Zuzana Storchova
contact affiliation	Molecular Genetics, TU Kaiserslautern
contact email	storchova@bio.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022660

PRIDE project URI

• PRIDE
  1. PXD022660
     1. Label: PRIDE project
     2. Name: The ER protein Ema19 facilitates the degradation of non-imported mitochondrial precursor proteins