PXD022652

PXD022652 is an original dataset announced via ProteomeXchange.

Title	A WDR35-dependent coatomer transports ciliary membrane proteins from the Golgi to the cilia
Description	Intraflagellar transport (IFT) is a highly conserved mechanism for motor-driven transport of cargo inside cilia. However, the mechanism of selective transport of cargo to cilia and entry across the diffusion barrier is not well understood. WDR35/IFT121 is a component of the IFT-A complex, best known for ciliary retrograde transport. Small Wdr35 mutant cilia are formed but fail to enrich in diverse classes of ciliary membrane proteins. Whilst the assembly of the IFT-B complex is unaffected, these exhibit retrograde trafficking defects in Wdr35 mutants. In contrast, the IFT-A peripheral components are degraded and core components remain stuck at the cilia base. The deep sequence homology and structural similarity of WDR35 and other IFT-As to the coatomer COPI proteins α and ß’, and accumulation of electron-light vesicles around Wdr35 mutant cilia, suggest that WDR35 is required to form coated vesicles delivering cargo from the Golgi necessary for cilia elongation. This is the first insitu proof towards a novel coatomer function for WDR35 and likely other IFT-A proteins in transporting ciliary membrane proteins from the Golgi.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-02_22:11:36.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimi Wills
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-20 07:35:01	ID requested
⏵ 1	2021-05-02 22:11:37	announced

Dataset with its publication pending

submitter keyword: cilia, WDR35, IFTA, ciliary membrane

Pleasantine Mill
contact affiliation	MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU
contact email	pleasantine.mill@igmm.ed.ac.uk
lab head
Jimi Wills
contact affiliation	University of Edinburgh
contact email	jimi.wills@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022652
     1. Label: PRIDE project
     2. Name: A WDR35-dependent coatomer transports ciliary membrane proteins from the Golgi to the cilia