PXD022644

PXD022644 is an original dataset announced via ProteomeXchange.

Title	First penicillin-binding protein occupancy patterns for 15 β-lactams and β-lactamase inhibitors in Mycobacterium abscessus
Description	Mycobacterium abscessus (Mab) causes serious infections that often require over 18 months of antibiotic combination therapy. With β lactam antibiotics being safe, double β-lactam and β-lactam/β-lactamase inhibitor combinations are of interest for improving treatment of Mab infections and minimizing toxicity. However, a mechanistic approach for building these combinations is lacking since little is known about which penicillin-binding protein (PBP) target receptors are inactivated by different β-lactams in Mab. This project aimed to identify PBPs in Mab and study the binding affinities of each of these PBPs with β-lactam antibiotics. These first PBP occupancy patterns in Mab provide a mechanistic foundation for selecting and optimizing safe and effective combination therapies with β-lactams.
HostingRepository	PRIDE
AnnounceDate	2020-12-01
AnnouncementXML	Submission_2020-11-30_23:09:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022644
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yinzhi Lang
SpeciesList	scientific name: Mycobacteroides abscessus ATCC 19977; NCBI TaxID: 561007;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-20 02:58:08	ID requested
1	2020-11-23 23:29:29	announced
⏵ 2	2020-11-30 23:09:01	announced	2020-12-01: Updated project metadata.

Sayed ARM, Shah NR, Basso KB, Kamat M, Jiao Y, Moya B, Sutaria DS, Lang Y, Tao X, Liu W, Shin E, Zhou J, Werkman C, Louie A, Drusano GL, Bulitta JB, -Lactamase Inhibitors in Mycobacterium abscessus. Antimicrob Agents Chemother, 65(1):(2020) [pubmed]

Sutaria DS, Moya B, Green KB, Kim TH, Tao X, Jiao Y, Louie A, Drusano GL, Bulitta JB, -Lactamase Inhibitors in Klebsiella pneumoniae. Antimicrob Agents Chemother, 62(6):(2018) [pubmed]

submitter keyword: Penicillin-binding proteins, Mycobacterium abscessus, beta-lactams, cell wall biosynthesis, PonA2, PonA1, PbpA

Jürgen B. Bulitta
contact affiliation	Department of Pharmacotherapy and Translational Research, College of Pharmacy, University of Florida, Orlando, FL
contact email	jbulitta@cop.ufl.edu
lab head
Yinzhi Lang
contact affiliation	Department of Pharmacotherapy and Translational Research, College of Pharmacy, University of Florida
contact email	y.lang@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD022644

PRIDE project URI

• PRIDE
  1. PXD022644
     1. Label: PRIDE project
     2. Name: First penicillin-binding protein occupancy patterns for 15 β-lactams and β-lactamase inhibitors in Mycobacterium abscessus