PXD022641

PXD022641 is an original dataset announced via ProteomeXchange.

Title	High fat diet mice intestinal epithelial exosomal proteins were analyzed by MSMS.
Description	High-fat diet (HFD) decreases insulin sensitivity. How high-fat diet causes insulin resistance is largely unknown. Here, we show that lean mice become insulin resistant after being administered exosomes isolated from the feces of obese mice fed a high-fat diet (HFD) or from human type II diabetic patients with diabetes. HFD altered the lipid composition of exosomes from predominantly PE in exosomes from lean animals (L-Exo) to PC in exosomes from obese animals (H-Exo). Mechanistically, we show that intestinal H-Exo is taken up by macrophages and hepatocytes, leading to inhibition of the insulin signaling pathway. Moreover, exosome-derived PC binds to and activates AhR, leading to inhibition of the expression of genes essential for activation of the insulin signaling pathway, including IRS-2, and its downstream genes PI3K and Akt. Together, our results reveal HFD-induced exosomes as potential contributors to the development of insulin resistance. Intestinal exosomes thus have potential as broad therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2021-03-23
AnnouncementXML	Submission_2021-03-23_12:02:21.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anil Kumar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-20 02:57:54	ID requested
⏵ 1	2021-03-23 12:02:23	announced

Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG, High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance. Nat Commun, 12(1):213(2021) [pubmed]

submitter keyword: L-Exo, H-Exo, Insulin resistance

Huang-Ge Zhang
contact affiliation	Department of Microbiology & Immunology School of Medicine University of Louisville CTRB, Room 309 Louisville, KY, USA 40202
contact email	h0zhan17@louisville.edu
lab head
Anil Kumar
contact affiliation	University of Louisville
contact email	a0kuma19@louisville.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022641

PRIDE project URI

• PRIDE
  1. PXD022641
     1. Label: PRIDE project
     2. Name: High fat diet mice intestinal epithelial exosomal proteins were analyzed by MSMS.