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PXD022639 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted mass spectrometry quantification of total soy protein residues from commercially processed ingredients for food allergen management
DescriptionSoybean is one of the most commonly allergenic foods in the U.S., and the ubiquity and variety of commercial soy ingredients in the food industry make it a challenging allergen to avoid. The processing methods used to produce soy-derived ingredients result in protein modifications that often substantially impact detection and quantification with commonly used antibody-based methods. This study aimed to develop a mass spectrometry (MS)-based method capable of quantifying commercially processed soy ingredients in food matrices. A quantification strategy using external standards with internal calibrants was developed and evaluated, resulting in the ultimate use of a matrix-independent standard curve of non-roasted soy flour with milk proteins as carrier proteins. The method performance was evaluated by quantifying six soy-derived ingredients in incurred food matrices. Out of the twelve ingredient-matrix combinations with 10 ppm incurred total soy protein, eight had maximum recoveries between 60-120%. Compared with three commercial ELISA kits, the MS method showed a substantial advantage in quantifying the highly processed soy proteins in food matrices.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterShimin Chen
SpeciesList scientific name: Glycine max; NCBI TaxID: 3847;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-19 12:13:33ID requested
12021-03-29 20:25:55announced
Publication List
Chen S, Yang C, Downs M, Targeted mass spectrometry quantification of total soy protein residues from commercially processed ingredients for food allergen management. J Proteomics, 239():104194(2021) [pubmed]
Keyword List
submitter keyword: Soybean
food allergen
mass spectrometry
Contact List
Melanie Downs
contact affiliationUniversity of Nebraska-Lincoln
contact emailmdowns2@unl.edu
lab head
Shimin Chen
contact affiliationUniversity of Nebraska-Lincoln
contact emailshimin@huskers.unl.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI