PXD022633

PXD022633 is an original dataset announced via ProteomeXchange.

Title	The effect of interferons on presentation of defective ribosome products as HLA peptides
Description	The class I major histocompatibility complex (MHC) bound peptides, produced from immature proteins that are degraded rapidly are called defective ribosome products (DRiPs). Such DRiPs are possibly involved with early alerting of the immune system about impeding infections, thus bringing about faster killing of infected cells before the viruses replicate. Interferons are a major group of cytokines, produced in response to viral infection. The interferons modulate the cellular metabolism and gene expression patterns and increase the expression and cell-surface presentation of the MHC molecules, helping this way coping with the viral infection. In this study, we evaluated whether the interferons also induce rapid degradation of cellular or viral proteins and produce DRiPs MHC-bound peptides to help the alert of the immune system. Cultured human breast cancer cells were treated with interferons and the cells were transferred to growth media containing heavy stable isotope labeled amino acids (dynamic-SILAC)in several time points a few hours after the interferons’ treatment. The rates of synthesis, degradation, and production of the cellular protein and their degradation products, the MHC peptides, were followed by LC-MS/MS analyses. Detection of large numbers of MHC peptides that incorporate the heavy amino acids into them, faster than their source proteins, indicated to us that not only DRiP-peptides are rather abundant among the MHC peptidome, but that the interferons increase significantly the presentation of DRiP-peptides. Importantly, much of this DRiPome derive from multi-subunit complexes, including the proteasomes and ribosomes, while the degradation of the standard protostome give rise to the immunoproteasome, which is thought to produce peptides that enhance the immune-response; the degradation of the ribosome subunits may aid in reducing the synthesis of viral infection
HostingRepository	PRIDE
AnnounceDate	2021-06-09
AnnouncementXML	Submission_2021-06-09_00:22:32.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dganit Melamed Kadosh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-19 03:33:05	ID requested
⏵ 1	2021-06-09 00:22:33	announced

Komov L, Melamed Kadosh D, Barnea E, Admon A, The Effect of Interferons on Presentation of Defective Ribosomal Products as HLA Peptides. Mol Cell Proteomics, 20():100105(2021) [pubmed]

submitter keyword: DRiPs, MHC, HLA peptides, IFNs

Arie Admon
contact affiliation	Faculty of Biology, Technion-Israel Institute of Technology
contact email	admon@technion.ac.il
lab head
Dganit Melamed Kadosh
contact affiliation	Technion
contact email	sdganit@tx.technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022633
     1. Label: PRIDE project
     2. Name: The effect of interferons on presentation of defective ribosome products as HLA peptides