PXD022626

PXD022626 is an original dataset announced via ProteomeXchange.

Title	An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus
Description	The virion proteins of Kaposi Sarcoma Associated Herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined detected 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via Ultra-High Resolution Qq-Time-Of-Flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins.
HostingRepository	PRIDE
AnnounceDate	2020-12-10
AnnouncementXML	Submission_2020-12-10_01:27:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022626
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	basir syed
SpeciesList	scientific name: Human herpesvirus 8 strain GK18; NCBI TaxID: 868565;
ModificationList	L-methionine sulfoxide; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-19 03:32:26	ID requested
⏵ 1	2020-12-10 01:27:30	announced

Nabiee R, Syed B, Ramirez Castano J, Lalani R, Totonchy JE, An Update of the Virion Proteome of Kaposi Sarcoma-Associated Herpesvirus. Viruses, 12(12):(2020) [pubmed]

submitter keyword: HHV8 Bottom-up Proteomics

Jennifer E. Totonchy
contact affiliation	Assistant Professor Biomedical and Pharmaceutical Sciences Department, ChapmanUniversity School of Pharmacy, Irvine,California, USA
contact email	totonchy@chapman.edu
lab head
basir syed
contact affiliation	chapman University school of pharmacy
contact email	bsyed@chapman.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022626
     1. Label: PRIDE project
     2. Name: An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus