PXD022622

PXD022622 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of C. glutamicum anaplerotic deletion mutants under defined D-glucose conditions
Description	Wild-type C. glutamicum ATCC 13032 is known to possess two enzymes with anaplerotic (C4-directed) carboxylation activity, namely phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx). On the other hand, C3-directed decarboxylation can be catalyzed by the three enzymes phosphoenolpyruvate carboxykinase (PEPCk), oxaloacetate decarboxylase (ODx), and malic enzyme (ME). The resulting high metabolic flexibility at the anaplerotic node compromises the unambigous determination of its carbon and energy flux in C. glutamicum wild type. To circumvent this problem we performed a comprehensive analysis of selected single or double deletion mutants in the anaplerosis of wild-type C. glutamicum under defined D-glucose conditions. By applying well-controlled lab-scale bioreactor experiments in combination with untargeted proteomics, quantitative metabolomics and whole-genome sequencing hitherto unknown, and sometimes counter-intuitive, genotype-phenotype relationships in these mutants could be unraveled. In comparison to the wild type the four mutants C. glutamiucm pyc, C. glutamiucmpyc odx, C. glutamiucm ppc pyc and C. glutamiucm pck showed lowered specific growth rates and D-glucose uptake rates, underlining the importance of PCx and PEPCk activity for a balanced carbon and energy flux at the anaplerotic node. Most interestingly, the strain C. glutamiucm ppc pyc could be evolved to grow on D-glucose as the only source of carbon and energy, whereas this combination was previously considered lethal. The prevented anaplerotic carboxylation activity of PEPCx and PCx was found in the evolved strain to be compensated by an up-regulation of the glyoxylate shunt, potentially in combination with the 2-methylcitrate cycle.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_14:27:34.099.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bianca Klein
SpeciesList	scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-19 03:31:54	ID requested
⏵ 1	2021-09-09 14:27:34	announced

Kappelmann J, Klein B, Papenfu, ß M, Lange J, Blombach B, Takors R, Wiechert W, Polen T, Noack S, Anaplerotic Deletion Mutants Under Defined d-Glucose Conditions. Front Bioeng Biotechnol, 8():602936(2020) [pubmed]

submitter keyword: Corynebacterium glutamicum, anaplerosis, phosphoenolpyruvate carboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxykinase, oxaloacetate decarboxylase, malic enzyme, methylcitrate cycle

Stephan Noack
contact affiliation	Forschungszentrum Juelich GmbH Institute of Bio- and Geosciences 1 Systems Biotechnology Group: Quantitative Microbial Phenotyping
contact email	s.noack@fz-juelich.de
lab head
Bianca Klein
contact affiliation	Forschungszentrum Juelich GmbH
contact email	b.klein@fz-juelich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022622

PRIDE project URI

• PRIDE
  1. PXD022622
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of C. glutamicum anaplerotic deletion mutants under defined D-glucose conditions