PXD022621

PXD022621 is an original dataset announced via ProteomeXchange.

Title	The UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
Description	The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in yeast and Ube2K in mammals exclusively generate polyubiquitin linked through lysine 48 (K48). Uniquely among E2 enzymes, Ubc1 and Ube2K harbor a Ub binding UBA domain with unknown function. We found that this UBA domain preferentially interacts with Ub chains linked through lysine 63 (K63). Based on structural modeling, in vitro ubiquitination experiments and NMR studies, we propose that the UBA domain aligns Ubc1 with K63-linked polyubiquitin and facilitates the selective assembly of K48/K63-branched Ub molecules. Genetic experiments link the activity of the UBA domain and hence the formation of this unusual Ub chain topology to the maintenance of cellular proteostasis.
HostingRepository	PRIDE
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-02_05:57:00.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik Zauber
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-19 03:31:51	ID requested
⏵ 1	2021-03-02 05:57:01	announced

Pluska L, Jarosch E, Zauber H, Kniss A, Waltho A, Bagola K, von Delbr, ü, ck M, L, ö, hr F, Schulman BA, Selbach M, D, ö, tsch V, Sommer T, The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains. EMBO J, 40(6):e106094(2021) [pubmed]

submitter keyword: Ubiquitin, branched, Yeast, UBC1, UBC4

Matthias Selbach
contact affiliation	Max Delbrück Center for Molecular Medicine in the Helmholtz Association
contact email	matthias.selbach@mdc-berlin.de
lab head
Henrik Zauber
contact affiliation	MDC Berlin-Buch
contact email	henrik.zauber@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022621
     1. Label: PRIDE project
     2. Name: The UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains