PXD022620

PXD022620 is an original dataset announced via ProteomeXchange.

Title	A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI-SNF complex component
Description	Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI-SNF family of chromatin remodelling complexes in an NCAPH2-independent manner.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-13_23:53:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-19 03:31:37	ID requested
⏵ 1	2020-12-13 23:53:15	announced

Dataset with its publication pending

submitter keyword: Protein interactions, condensin, NCAPH2

Jyoti Choudhary
contact affiliation	The Institute of Cancer Research, London
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	The Institute of Cancer Research
contact email	james.wright@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022620
     1. Label: PRIDE project
     2. Name: A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI-SNF complex component