PXD022607 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The PI3Kδ interactome in naïve and activated mouse CD4+ T cells |
Description | Phosphoinositide 3-kinases (PI3Ks) play a central role in adaptive immunity by transducing signals from the T cell antigen receptor (TCR) via production of PIP3. PI3Kδ is a heterodimer composed of a p110δ catalytic subunit associated with a p85α or p85β regulatory subunit and is preferentially engaged by the TCR upon T cell activation. The molecular mechanisms leading to PI3Kδ recruitment and activation at the TCR signalosome remain unclear. In this study, we have used affinity purification coupled with quantitative mass spectrometry to uncover the p110δ interactome in primary CD4+ T cells. Moreover, we have determined how the PI3Kδ interactome changes upon the differentiation of small naïve T cells into T cell blasts expanded in the presence of IL-2. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-08 |
AnnouncementXML | Submission_2021-12-07_03:40:48.537.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daisy Luff |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated N-terminal; monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent N6-acylated lysine |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-18 08:03:58 | ID requested | |
1 | 2021-03-07 22:32:49 | announced | |
⏵ 2 | 2021-12-07 03:40:49 | announced | 2021-12-07: Updated publication reference for PubMed record(s): 33763075. |
Publication List
Luff DH, Wojdyla K, Oxley D, Chessa T, Hudson K, Hawkins PT, Stephens LR, Barry ST, Okkenhaug K, T Cells. Front Immunol, 12():631271(2021) [pubmed] |
Keyword List
submitter keyword: PI3K, PI3Kδ, T-cells, TCR, CD4+, interactome, AP-MS, nLC-MS/MS, TMT, 10-plex, mouse |
Contact List
Klaus Okkenhaug |
contact affiliation | Department of Pathology, University of Cambridge, Cambridge, UK |
contact email | ko256@cam.ac.uk |
lab head | |
Daisy Luff |
contact affiliation | The Francis Crick Institute, London, UK |
contact email | daisy.luff@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022607
- Label: PRIDE project
- Name: The PI3Kδ interactome in naïve and activated mouse CD4+ T cells