PXD022605

PXD022605 is an original dataset announced via ProteomeXchange.

Title	Scaling of cellular processes with ploidy
Description	While most eukaryotic cells are diploid, with two chromosome sets, variances in ploidy are common. Despite the relative prevalence of ploidy changes and their relevance for pathology and evolution, a complete picture of consequences of altered ploidy is missing. We analyzed transcriptome and proteome changes in budding yeast Saccharomyces cerevisiae from haploid to tetraploid and found that the mRNA and protein abundance increases linearly with ploidy, but does not double with doubling the DNA content. Besides this linear increase, we found that pathways related to mitochondria and to cytoplasmic ribosomes and translation are differentially regulated. Indeed, with increasing ploidy the cells reduce mitochondrial content and this effect can be rescued by antioxidants. Moreover, cells of higher ploidy reduce their ribosome content while maintaining constant translational output. We show that this is an active process regulated via the Tor1 and Sch9 kinases and a transcriptional corepressor of rDNA transcription, Tup1. Similarly, human tetraploid cells downregulate their ribosome content via Tle1, a Tup1 homolog, demonstrating that the proteome remodeling is a conserved response to increased ploidy.
HostingRepository	PRIDE
AnnounceDate	2022-08-15
AnnouncementXML	Submission_2022-08-15_00:33:23.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Menges
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-18 08:03:55	ID requested
⏵ 1	2022-08-15 00:33:23	announced

Dataset with its publication pending

submitter keyword: Yeast, Ploidy, LC-MSMS, SILAC

Prof. Dr. Zuzana Storchova
contact affiliation	University Kaiserslautern
contact email	storchova@bio.uni-kl.de
lab head
Paul Menges
contact affiliation	Technische Universität Kaiserslautern
contact email	pmenges@bio.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD022605

PRIDE project URI

• PRIDE
  1. PXD022605
     1. Label: PRIDE project
     2. Name: Scaling of cellular processes with ploidy