PXD022597

PXD022597 is an original dataset announced via ProteomeXchange.

Title	Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response
Description	Candida glabrata is a clinically relevant human pathogen with ability to form high recalcitrant biofilms that contribute to the establishment and persistence of infection. A defining trait of biofilms is the auto-produced matrix, which is suggested to have structural, virulent and protective roles. Thus, elucidation of matrix components, their function and modulation by host environment is crucial to disclose their role in C. glabrata pathogenesis. As a major step toward this end, this study aimed to reveal, for the first time, the matrix proteome of C. glabrata biofilms, to characterize it with bioinformatic tools and to study its modulation by the environmental pH (acidic and neutral). The results showed the presence of several pH-specific matrix proteins (51 acidic- and 206 neutral-specific) and also proteins commonly found at both pH conditions (236). Of note, several proteins related to β-glucan metabolism, which have a potential role in the delivery/organization of matrix glucans, were found in both conditions but in much higher quantity under the neutral environment. Additionally, proteins with roles in adhesion, including epithelial adhesins, and virulence-related, including yapsins, were found among pH-specific and non-specific matrix proteins. Importantly, several proteins seem to have a non-canonical secretion pathway and Pdr1 was found to be a potential regulator of matrix proteome. Overall, this study indicates a relevant impact of environmental cues in the matrix proteome and provides a unique resource for further functional investigation of matrix proteins, contributing to the identification of potential targets for the development of new therapies against C. glabrata biofilms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:29.417.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022597
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Candida glabrata (Yeast) (Torulopsis glabrata); NCBI TaxID: 5478;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-18 01:40:31	ID requested
1	2024-02-22 08:11:44	announced
⏵ 2	2024-10-22 06:31:30	announced	2024-10-22: Updated project metadata.

10.1042/bcj20200844;

10.6019/PXD022597;

Gon, ç, alves B, Azevedo N, Os, ó, rio H, Henriques M, Silva S, Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response. Biochem J, 478(4):961-974(2021) [pubmed]

submitter keyword: biofilm secretome, environmental pH, adhesins, β-glucans,candidiasis, yapsins

Hugo Osório
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto, Portugal
contact email	hosorio@i3s.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD022597

PRIDE project URI

• PRIDE
  1. PXD022597
     1. Label: PRIDE project
     2. Name: Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response