PXD022581

PXD022581 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry data for Leishmania major proteins obtained by immunoprecipitation with anti-PUF1, anti-PUF3 or pre-immunization antibodies.
Description	This project was aimed to the characterization of proteins interacting with the RNA binding proteins PUF1 and PUF3 of Leishmania major (Friedlin strain). For this purpose an immunoprecipitation (IP) strategy was followed. Firstly, protein extracts from L. major promastigotes were incubated with specific antibodies, raised in rabbits, against recombinant proteins PUF1 or PUF3. In parallel, a control IP using a pre-immune serum was performed. After affinity purification of the antibody-protein complexes, proteins were analyzed by Reverse phase- Liquid Chomatography/Mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2021-05-17
AnnouncementXML	Submission_2021-05-16_22:57:37.758.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022581
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jose María Requena
SpeciesList	scientific name: Leishmania major; NCBI TaxID: 5664;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 03:56:09	ID requested
⏵ 1	2021-05-16 22:57:38	announced

Dataset with its publication pending

submitter keyword: Leishmania major

promastigote

PUF1

PUF3

immunoprecipitation

mass spectrometry

Jose M. Requena
contact affiliation	Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid
contact email	jmrequena@cbm.csic.es
lab head
Jose María Requena
contact affiliation	Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid
contact email	jmrequena@cbm.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022581
     1. Label: PRIDE project
     2. Name: Mass spectrometry data for Leishmania major proteins obtained by immunoprecipitation with anti-PUF1, anti-PUF3 or pre-immunization antibodies.