PXD022577

PXD022577 is an original dataset announced via ProteomeXchange.

Title	Proteomic Quantification of Lysine Acetylation in mouse hippocampus
Description	Major depressive disorder (MDD) is a leading cause of disability around the world and contributes greatly to the global burden of disease. Mounting evidence suggests that gut microbiota dysbiosis may be involved in the pathophysiology of MDD through the microbiota–gut–brain axis. Recent research suggests that epigenetic modifications might relate to depression. However, our knowledge of the role of epigenetics in host–microbe interactions remains limited. In the present study, we used a combination of affinity enrichment and high-resolution liquid chromatography tandem mass spectrometry analysis to identify hippocampal acetylated proteins in germ-free(GF) and specific pathogen-free(SPF) mice. In total, 986 lysine acetylation sites in 543 proteins were identified, of which 747 sites in 427 proteins were quantified. Motif analysis identified several conserved sequences surrounding the acetylation sites, including D*Kac, DKac, KacY, KacD, and D**Kac. Gene ontology annotations revealed that these differentially expressed acetylated proteins were involved in multiple biological functions and mainly located in mitochondria. In addition, pathway enrichment analysis showed that oxidative phosphorylation and the tricarboxylic acid (TCA) cycle II (eukaryotic), both of which are exclusively localized to the mitochondria, were the primarily disturbed functions. Taken together, this study indicates that lysine acetylation changes may play a pivotal role in the mitochondrial dysfunction by which gut microbiota regulate brain function and behavioral phenotypes.
HostingRepository	PRIDE
AnnounceDate	2021-03-09
AnnouncementXML	Submission_2021-03-08_22:25:11.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Ying
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 01:54:07	ID requested
⏵ 1	2021-03-08 22:25:12	announced

Dataset with its publication pending

submitter keyword: Depression, gut microbiota, lysine acetylation, germ-free, specific pathogen-free

xie peng
contact affiliation	NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China;
contact email	xiepeng@cqmu.edu.cn
lab head
Yu Ying
contact affiliation	Chongqing Medical University
contact email	1262972437@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022577
     1. Label: PRIDE project
     2. Name: Proteomic Quantification of Lysine Acetylation in mouse hippocampus