PXD022570

PXD022570 is an original dataset announced via ProteomeXchange.

Title	Signal Recognition Particle RNA Contributes to Oxidative Stress Response in Deinococcus radiodurans by Modulating Catalase Localization
Description	The proper functioning of many proteins requires their transport to the correct cellular compartment or their secretion. Signal recognition particle (SRP) is a major protein transport pathway responsible for the co-translational movement of integral membrane proteins as well as periplasmic proteins. Deinococcus radiodurans is a ubiquitous bacterium that expresses a complex phenotype of extreme oxidative stress resistance, which is mediated by proteins involved in DNA repair, metabolism, gene regulation and antioxidant defence. These proteins are located either extracellularly or subcellularly, but the molecular mechanism of protein localization in D. radiodurans to manage oxidative stress response remains unexplored. In this study, we characterized the SRP complex in D. radiodurans R1 and showed that the knockdown of the SRP RNA (Qpr6) reduced bacterial survival under hydrogen peroxide (H2O2) and growth under chronic ionizing radiation (CIR). Through LC-MS/MS analysis, we detected 162 proteins in the periplasm of wild-type D. radiodurans, of which the transport of 65 of these proteins to the periplasm was significantly reduced in the Qpr6 knockdown strain. Through Western blotting, we further demonstrated the localization of the catalases in D. radiodurans, DR_1998 (KatE1) and DR_A0259 (KatE2), in both the cytoplasm and periplasm, and showed that the accumulation of KatE1 and KatE2 in the periplasm was reduced in the SRP-defective (Qpr6 knockdown) strain.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:49.257.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022570
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	RUNHUA HAN
SpeciesList	scientific name: Deinococcus radiodurans ATCC 13939; NCBI TaxID: 1408434;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 01:53:26	ID requested
1	2023-03-10 18:39:31	announced
⏵ 2	2023-11-14 08:28:50	announced	2023-11-14: Updated project metadata.

10.6019/PXD022570;

Han R, Fang J, Jiang J, Gaidamakova EK, Tkavc R, Daly MJ, Contreras LM, by Modulating Catalase Localization. Front Microbiol, 11():613571(2020) [pubmed]

submitter keyword: signal recognition particle, protein transport, periplasm, small non-coding RNA, catalase,Deinococcus radiodurans, oxidative stress

Lydia M. Contreras
contact affiliation	1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA
contact email	lcontrer@che.utexas.edu
lab head
RUNHUA HAN
contact affiliation	Univesity of Texas at Austin
contact email	runhuahan@utexas.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022570
     1. Label: PRIDE project
     2. Name: Signal Recognition Particle RNA Contributes to Oxidative Stress Response in Deinococcus radiodurans by Modulating Catalase Localization