PXD022569

PXD022569 is an original dataset announced via ProteomeXchange.

Title	Phytophthora detection in planta
Description	Phytophthora is arguably one of the most damaging genera of plant pathogens and is recognized as a threat to a broad spectrum of plant species. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for its effective control and of paramount importance in reducing the economic and ecological impact. The increasing sensitivity of the mass spectrometry-based detection indicates that a protein-based analysis may soon complement routine molecular techniques for pathogen detection. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened, and species-specific peptide patterns were identified. Next, Phytophthora proteins were detected in planta, employing model plants S. tuberosum and H. vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection, the comparison between qPCR and protein data highlighted more than 300 protein markers correlated positively with the amount of P. infestans DNA. Finally, the analysis of P. palmivora response in barley proteome and metabolome revealed significant alterations in primary metabolism, providing the first insight into molecular mechanisms behind this biotic interaction.
HostingRepository	PRIDE
AnnounceDate	2020-12-15
AnnouncementXML	Submission_2020-12-15_04:57:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 01:53:23	ID requested
⏵ 1	2020-12-15 04:57:13	announced

Dataset with its publication pending

submitter keyword: Phytophthora, proteomics, P. palmivora, P. infestans, Solanum tuberosum, Hordeum vulgare

Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022569

PRIDE project URI

• PRIDE
  1. PXD022569
     1. Label: PRIDE project
     2. Name: Phytophthora detection in planta