PXD022566

PXD022566 is an original dataset announced via ProteomeXchange.

Title	Human EDEM2 proteomics and glycoproteomics datasets
Description	The endoplasmic reticulum (ER) homeostasis is maintained by redirecting misfolded or unfolded polypeptide products towards the endoplasmic reticulum associated degradation (ERAD), where proteins are sent to degradation in the ubiquitin-proteasome system. One active–player in this process, is EDEM2 (ER degradation-enhancing alpha-mannosidase-like protein 2), presumably to be one of the first ER mannosidases to act in the early steps of ERAD. Here, we provide a full data set encoding a proteomic, affinity-proteomic and glycoproteomic analysis of melanoma cells with altered expression level of EDEM2. Our affinity-proteomics data are complemented by molecular mass separation of the identified complexes in sucrose fractionation experiments, while the glycoproteomic results allowed the identification of new glycosylation sites regulated by EDEM2 altered expression level. These results were also validated by alternative biochemical methods.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_04:31:49.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Munteanu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylhexosaminylated; HexNAc1dHex1; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 09:43:06	ID requested
⏵ 1	2022-04-13 04:31:49	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, ERAD, EDEM2, melanoma, glycoproteomics, affinity proteomics

Stefana M. Petrescu
contact affiliation	Department of Molecular Cell Biology, Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest 17, Romania
contact email	stefana.petrescu@biochim.ro
lab head
Cristian Munteanu
contact affiliation	Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	cristian.v.a.munteanu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD022566

PRIDE project URI

• PRIDE
  1. PXD022566
     1. Label: PRIDE project
     2. Name: Human EDEM2 proteomics and glycoproteomics datasets