PXD022565

PXD022565 is an original dataset announced via ProteomeXchange.

Title	Transfection with Glucuronidase A affects general proteome pattern in Giardia lamblia trophozoites
Description	Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern. Using shotgun mass spectrometry, we compare the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA). Besides GusA, which is detected in the transfected trophozoites only, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. In particular, transfection induces antigenic variation. Since transfection causing stable expression affects the proteome pattern, transfection experiments should take into account this effect. Due to a unique peptide panel, GusA is an example for a suitable internal standard for experiments involving transfected cells.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_14:44:02.327.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Giardia intestinalis assemblage B; NCBI TaxID: 1394984;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 08:01:39	ID requested
⏵ 1	2021-03-04 14:44:03	announced

Heller M, Braga S, M, ü, ller N, M, ü, ller J, Trophozoites. Front Cell Infect Microbiol, 10():602756(2020) [pubmed]

submitter keyword: controls and standards in proteomic experimental procedures

model organisms

reverse genetics

untargeted proteomics

Norbert Müller
contact affiliation	Institute of parasitology Vetsuisse University of Bern
contact email	norbert.mueller@vetsuisse.unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022565
     1. Label: PRIDE project
     2. Name: Transfection with Glucuronidase A affects general proteome pattern in Giardia lamblia trophozoites