PXD022562

PXD022562 is an original dataset announced via ProteomeXchange.

Title	Interaction of Chinese cabbage (Brassica rapa ssp. pekinensis) cell wall proteins with mustard leaf beetle (Phaedon cochleariae) polygalacturonase family members
Description	Plants possess various defense strategies to counter attacks from microorganisms or herbivores. For example, plants reduce the cell-wall-macerating activity of pathogen- or insect-derived polygalacturonases (PGs) by expressing PG-inhibiting proteins (PGIPs). PGs and PGIPs belong to multi-gene families believed to have been shaped by an evolutionary arms race. The mustard leaf beetle Phaedon cochleariae expresses both active PGs and catalytically inactive PG pseudoenzymes. Previous studies demonstrated that (i) PGIPs target beetle PGs and (ii) the role of PG pseudoenzymes remains elusive, despite having been linked to the pectin degradation pathway. For further insight into the interaction between plant PGIPs and beetle PG family members, we combined affinity purification with proteomics and gene expression analyses, and identified novel inhibitors of beetle PGs from Chinese cabbage (Brassica rapa ssp. pekinensis). A beetle PG pseudoenzyme was not targeted by PGIPs, but instead interacted with PGIP-like proteins. Phylogenetic analysis revealed that PGIP-like proteins clustered apart from classical PGIPs but together with proteins, which have been involved in developmental processes. Our results indicate that PGIP-like proteins represent not only interesting novel PG inhibitor candidates in addition to classical PGIPs, but also fascinating new players in the arms race between herbivorous beetles and plant defenses.
HostingRepository	PRIDE
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2021-06-06_22:39:42.398.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022562
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wiebke Haeger
SpeciesList	scientific name: Phaedon cochleariae; NCBI TaxID: 80249; scientific name: Brassica rapa subsp. pekinensis; NCBI TaxID: 51351;
ModificationList	iodoacetamide derivatized residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 07:17:24	ID requested
⏵ 1	2021-06-06 22:39:42	announced

Dataset with its publication pending

submitter keyword: beetle, polygalacturonase, polygalacturonase-inhibiting proteins, cell wall proteins, Chinese cabbage, cell wall-degrading enzymes, LC-MS/MS, pull-down

Yannick Pauchet
contact affiliation	Max Planck Institut for Chemical Ecology, Department of Entomology, Project Group Molecular Biology of the Insect Digestive System, Germany
contact email	ypauchet@ice.mpg.de
lab head
Wiebke Haeger
contact affiliation	Max Planck Institute for Chemical Ecology
contact email	wihaeger@ice.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022562
     1. Label: PRIDE project
     2. Name: Interaction of Chinese cabbage (Brassica rapa ssp. pekinensis) cell wall proteins with mustard leaf beetle (Phaedon cochleariae) polygalacturonase family members