PXD022558 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic TandemMassTag analysis of intestinal epithelial cells (IEC) deleted for both Hdac1 and Hdac2 |
Description | We have performed quantitative proteomic TandemMassTag to investigate proteomic changes after deletion of epigenetic eraser genes Hdac1 and Hdac2 in intestinal epithelial cells. Both HDAC1 and HDAC2 are epigenetic erasers that drive specific and redundant gene expression patterns, in part by removing acetyl groups on histones. Deletion of these Hdac in intestinal epithelial cell (IEC) in vivo alters intestinal homeostasis, dependent on the Hdac deleted and the level of expression of both. To determine the specific IEC function of HDAC1 and HDAC2, we have performed transcriptomic and quantitative proteomic approaches on IEC deficient in Hdac1 and Hdac2. We have defined changes in both mRNA and protein expression patterns affecting IEC differentiation. We have identified IEC Hdac1- and Hdac2-dependent common as well as specific pathways and biological processes. These findings uncover unrecognized similarities and differences between Hdac1 and Hdac2 in IEC. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_13:19:58.309.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dominique Levesque |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-16 03:39:51 | ID requested | |
⏵ 1 | 2021-09-09 13:19:59 | announced | |
Publication List
Gonneaud A, Turgeon N, Boisvert FM, Boudreau F, Asselin C, -Intestinal Epithelial Cell-Deficient Mice. Cells, 10(2):(2021) [pubmed] |
Keyword List
submitter keyword: Intestinal epithelial cell, HDAC, proteome |
Contact List
Claude Asselin |
contact affiliation | Claude Asselin, Université de Sherbrooke, Department of immunology and Cell Biology, Faculty of medicine and Health Sciences, Université de Sherbrooke (lab head) |
contact email | claude.asselin@USherbrooke.ca |
lab head | |
Dominique Levesque |
contact affiliation | University of Sherbrooke, FM Boisvert lab |
contact email | dominique.levesque@usherbrooke.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD022558
- Label: PRIDE project
- Name: Quantitative proteomic TandemMassTag analysis of intestinal epithelial cells (IEC) deleted for both Hdac1 and Hdac2