PXD022546

PXD022546 is an original dataset announced via ProteomeXchange.

Title	Mannose Receptor glycoprotein ligands in A549 cells
Description	The macrophage mannose receptor (CD206, MR) is an endocytic lectin receptor which plays an important role in homeostasis and innate immunity. The MR contains C-type lectin domains (CTLD) whose specificity for endogenous glycans and glycoprotein ligands have not been well studied. To gain more insights into the recognition by the MR, we used the murine MR CTLD 4-7 coupled to the Fc-part of IgG (MR-Fc) to investigate its glycan and glycoprotein recognition. As lung cancer tissue and the lung cancer cell line A549 showed intense MR-Fc binding, we further investigated the MR glycoprotein ligands in those cells by immunoprecipitation and glycoproteomic analysis. All enriched glycoproteins, of which 42 were identified, contained pauci- or oligomannose N-glycans, confirming the microarray results. Our study demonstrates that the MR CTLD4-7 is highly selective for pauci- and oligomannosidic N-glycans, structures that are often elevated in tumor cells, and suggest a potential role for the MR in tumor biology.
HostingRepository	PRIDE
AnnounceDate	2022-06-21
AnnouncementXML	Submission_2022-06-21_06:31:22.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathrin Stavenhagen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 03:38:43	ID requested
⏵ 1	2022-06-21 06:31:23	announced

Stavenhagen K, Laan LC, Gao C, Mehta AY, Heimburg-Molinaro J, Glickman JN, van Die I, Cummings RD, Tumor cells express pauci- and oligomannosidic N-glycans in glycoproteins recognized by the mannose receptor (CD206). Cell Mol Life Sci, 78(14):5569-5585(2021) [pubmed]

Lecker LSM, Berlato C, Maniati E, Delaine-Smith R, Pearce OMT, Heath O, Nichols SJ, Trevisan C, Novak M, McDermott J, Brenton JD, Cutillas PR, Rajeeve V, Hennino A, Drapkin R, Loessner D, Balkwill FR, TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer. Cancer Res, 81(22):5706-5719(2021) [pubmed]

submitter keyword: glycoproteomics, mannose receptor, A549, LC-MS

Richard D. Cummings
contact affiliation	Harvard Medical School Beth Israel Deaconess Medical Center Director, HMS Center for Glycoscience Director, National Center for Functional Glycomics CLS 11087 - 3 Blackfan Circle Boston, MA 02115, USA
contact email	rcummin1@bidmc.harvard.edu
lab head
Kathrin Stavenhagen
contact affiliation	Beth Israel Deaconess Medical Center, Harvard Medical School
contact email	kathrin.stavenhagen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD022546

PRIDE project URI

• PRIDE
  1. PXD022546
     1. Label: PRIDE project
     2. Name: Mannose Receptor glycoprotein ligands in A549 cells