PXD022539

PXD022539 is an original dataset announced via ProteomeXchange.

Title	Cold-induced phosphorylational sites analysis of protein kinase OST1
Description	For the analysis of phosphorylation sites of OST1, 8 g pSuper:OST1-Myc plants materials, treated with 4oC for 0 or 2 h, were used for protein extraction and immunoprecipitated with anti-Myc agarose. Then OST1-Myc proteins were eluted with 0.1 M glycine solution (pH 2.8) and mixed with 1 M Tris solution to ensure the pH of OST1-Myc is 7.5. After the concentration of PP2CG1 proteins with 30K-ultrafiltration tubes, phosphatase assay was performed: OST1-Myc was treated with GST-PP2CG1 for 0 or 60 min under 30oC. Then, the samples were digested with trypsin using FASP method. The collected solution containing digested peptide were lyophilized for phospho-peptides enrichment, which was performed using TiO2-tips (Thermo Scientific, #A32992) according to the manufacture’s protocol. The phosphor-peptides were resuspended in 0.1% FA for nano LC-MS analysis.
HostingRepository	iProX
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2021-04-20_01:22:52.047.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Lv
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-15 19:31:59	ID requested
⏵ 1	2021-04-20 01:22:52	announced

submitter keyword: phospho-sites, OST1, Cold-induced

Shuhua Yang
contact affiliation	China Agricultural University
contact email	yangshuhua@cau.edu.cn
lab head
Jian Lv
contact affiliation	China Agricultural University
contact email	734999889@qq.com
dataset submitter

iProX dataset URI