PXD022529 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid |
Description | Abstract Background: Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Methods: Samples of aqueous humor were collected from pediatric and adult cataract patients and underwent mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ontology analysis was performed using WikiPaths for each cataract subtype. Results: Seven pediatric patients and ten adult patients were included in the study. Of the pediatric samples, all seven (100%) were male, three (43%) had traumatic cataracts, two (29%) had congenital cataracts, and two (29%) had posterior polar cataracts. Of the adult patients, seven (70%) were female and seven (70%) had predominantly nuclear sclerotic cataracts. 128 proteins were upregulated in the pediatric samples and 127 proteins were upregulated in the adult samples, with 75 proteins shared by both groups. Gene ontology analysis identified inflammatory and oxidative stress pathways as upregulated in pediatric cataracts. Conclusions: Inflammatory and oxidative stress mechanisms may be involved in pediatric cataract formation and warrant further investigation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:54:43.605.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Donald Wolfgeher |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-13 08:00:25 | ID requested | |
1 | 2023-07-20 10:41:52 | announced | |
2 | 2023-11-14 09:02:25 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:54:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
Theophanous CN, Wolfgeher DJ, Farooq AV, Hilkert Rodriguez S, Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid. Int J Mol Sci, 24(10):(2023) [pubmed] |
10.3390/ijms24109040; |
Keyword List
submitter keyword: Q-Exactive,pediatric cataracts |
proteomics |
aqueous humor, LFQ |
Contact List
Dr. Sarah Hilkert Rodrigue, MD, MPH |
contact affiliation | Department of Ophthalmology and Visual Science, University of Chicago Medical Center, Chicago, IL. |
contact email | srodriguez5@bsd.uchicago.edu |
lab head | |
Donald Wolfgeher |
contact affiliation | University of Chicago |
contact email | donw@uchicago.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022529
- Label: PRIDE project
- Name: Biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid