PXD022529

PXD022529 is an original dataset announced via ProteomeXchange.

Title	Biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid
Description	Abstract Background: Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Methods: Samples of aqueous humor were collected from pediatric and adult cataract patients and underwent mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ontology analysis was performed using WikiPaths for each cataract subtype. Results: Seven pediatric patients and ten adult patients were included in the study. Of the pediatric samples, all seven (100%) were male, three (43%) had traumatic cataracts, two (29%) had congenital cataracts, and two (29%) had posterior polar cataracts. Of the adult patients, seven (70%) were female and seven (70%) had predominantly nuclear sclerotic cataracts. 128 proteins were upregulated in the pediatric samples and 127 proteins were upregulated in the adult samples, with 75 proteins shared by both groups. Gene ontology analysis identified inflammatory and oxidative stress pathways as upregulated in pediatric cataracts. Conclusions: Inflammatory and oxidative stress mechanisms may be involved in pediatric cataract formation and warrant further investigation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:54:43.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-13 08:00:25	ID requested
1	2023-07-20 10:41:52	announced
2	2023-11-14 09:02:25	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:54:45	announced	2024-10-22: Updated project metadata.

Theophanous CN, Wolfgeher DJ, Farooq AV, Hilkert Rodriguez S, Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid. Int J Mol Sci, 24(10):(2023) [pubmed]

10.3390/ijms24109040;

submitter keyword: Q-Exactive,pediatric cataracts

proteomics

aqueous humor, LFQ

Dr. Sarah Hilkert Rodrigue, MD, MPH
contact affiliation	Department of Ophthalmology and Visual Science, University of Chicago Medical Center, Chicago, IL.
contact email	srodriguez5@bsd.uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022529
     1. Label: PRIDE project
     2. Name: Biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid