PXD022527

PXD022527 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics characterization of proteins modified by very long chain fatty acids (VLCFAs) under necroptosis
Description	Necroptosis is a caspase-independent form of regulated cell death that is characterized by membrane permeabilization and rupture. This membrane rupture is responsible for the inflammatory properties of necroptosis and is critical for disease states involving this process. In efforts to understand how lipids might contribute to necroptosis, we previously showed that saturated very long chain fatty acids (VLCFAs) are functionally involved in this process, potentially through protein fatty acylation. Here we define the scope of protein acylation by saturated VLCFAs during necroptosis using a clickable lipid analog and IonStar quantitative proteomics.As a result, a total of 1672 proteins were quantified with high precision and no missing data across samples in the same condition. 1267 were detected with higher abundances in control cells and 405 were detected with higher abundance in necroptotic conditions.
HostingRepository	PRIDE
AnnounceDate	2021-05-26
AnnouncementXML	Submission_2021-05-25_22:08:46.167.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shichen Shen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-13 08:00:23	ID requested
1	2021-05-25 06:46:43	announced
⏵ 2	2021-05-25 22:08:46	announced	2021-05-26: Updated project metadata.

10.1016/J.CHEMBIOL.2021.03.012;

Pradhan AJ, Lu D, Parisi LR, Shen S, Berhane IA, Galster SL, Bynum K, Monje-Galvan V, Gokcumen O, Chemler SR, Qu J, Kay JG, Atilla-Gokcumen GE, Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis. Cell Chem Biol, 28(9):1298-1309.e7(2021) [pubmed]

submitter keyword: IonStar proteomics acylation necroptosis

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences School of Pharmacy and Pharmaceutical Sciences University at Buffalo, State University of New York
contact email	junqu@buffalo.edu
lab head
Shichen Shen
contact affiliation	University at Buffalo
contact email	shichens@buffalo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD022527

PRIDE project URI

• PRIDE
  1. PXD022527
     1. Label: PRIDE project
     2. Name: Quantitative proteomics characterization of proteins modified by very long chain fatty acids (VLCFAs) under necroptosis