PXD022498

PXD022498 is an original dataset announced via ProteomeXchange.

Title	Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm
Description	Meta-proteomics analysis approach in the application of biogas production from anaerobic digestion has many advantages that has not been fully uncovered yet. This study aims to investigate biogas production from a stable 2-stage chicken manure fermentation system in chemical and biological perspective. The diversity and functional protein changes from the 1st stage to 2nd stage is a good indication to expose the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explain the causation of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, the hydrogenotrophic methanogenic pathway is adopted as indicative of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomic analysis, this study could provide both species names of microorganism and enzymes to directly point the generation pathway of methane and carbon dioxide in investigating biogas production of chicken manure.
HostingRepository	PRIDE
AnnounceDate	2021-02-08
AnnouncementXML	Submission_2021-02-07_23:32:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tze-Kang Lam
SpeciesList	scientific name: Methanoculleus sp.; NCBI TaxID: 90427; scientific name: Lactobacillus crispatus EX533959VC03; NCBI TaxID: 858653; scientific name: Clostridum phage CDHM6; NCBI TaxID: 2228361;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-13 03:12:27	ID requested
1	2021-02-07 23:13:10	announced
2	2021-02-07 23:16:24	announced	2021-02-08: Updated project metadata.
⏵ 3	2021-02-07 23:32:20	announced	2021-02-08: Updated project metadata.

Dataset with its publication pending

submitter keyword: Meta-Proteomics Analysis

Chicken Manure

Metabolic Pathway

2-stage Fermentation System

Ammonia Inhibition

Shih-Hsiung Wu
contact affiliation	128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan, IBC R712
contact email	shwu@gate.sinica.edu.tw
lab head
Tze-Kang Lam
contact affiliation	Institute of Biological Chemistry, Academia Sinica
contact email	ltzekang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022498

PRIDE project URI

• PRIDE
  1. PXD022498
     1. Label: PRIDE project
     2. Name: Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm