PXD022480

PXD022480 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of two RNA-binding proteins, RBP23 and DRBD2, partners of different Poly(A) Binding proteins (PABP) homologues in Leishmania infantum
Description	RNA binding proteins (RBP) have multiple roles associated with mRNA stability and translation. In the Leishmania genus, three Poly(A) Binding Proteins (PABP) were encoded with yet undefined functional distinctions but previous studies of mass-spectrometry identified RBPs differentially associated with PABP1 and PABP2, including RBP23 and DRBD2, respectively. Further characterization of both RBPs were performed through co-immunoprecipitation of proteins with HA-tagged RBP23 and DRBD2 from Leishmania infantum cytoplasmic extract cells, combined to mass spectrometry analysis. The analysis confirmed a strong association between RBP23 and PABP1, as well as with the EIF4E4/EIF4G3, a typical translation initiation complex, while DRBD2 was found to be more associated with PABP2 and a large number of different protein partners. Our studies expand the knowledge about RBPs function and their partners during Leishmania mRNA translation/metabolism.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_05:18:59.856.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludmila Arruda de Assis
SpeciesList	scientific name: Leishmania infantum JPCM5; NCBI TaxID: 435258;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-12 01:56:03	ID requested
⏵ 1	2021-11-02 05:19:00	announced

Dataset with its publication pending

submitter keyword: Leishmania, RBP23, DRBD2

Osvaldo Pompílio de Melo Neto
contact affiliation	Department of Microbiology, Aggeu Magalhaes Institute, Brazil
contact email	opmn@cpqam.fiocruz.br
lab head
Ludmila Arruda de Assis
contact affiliation	Instituto Aggeu Magalhães - Fiocruz Pernambuco
contact email	ludmila18arruda@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD022480

PRIDE project URI

• PRIDE
  1. PXD022480
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of two RNA-binding proteins, RBP23 and DRBD2, partners of different Poly(A) Binding proteins (PABP) homologues in Leishmania infantum