PXD022476

PXD022476 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantification of mitochondrial translation by pulse SILAC
Description	We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide a global picture of (post-)translational regulation in mitochondria. Replicate 1: H-CRP_M-DMSO, Replicate 2: M-CRP_H-DMSO.
HostingRepository	jPOST
AnnounceDate	2022-12-28
AnnouncementXML	Submission_2024-03-12_19:38:07.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-11 17:18:52	ID requested
1	2022-12-27 12:42:35	announced
⏵ 2	2024-03-12 19:38:08	announced	2024-03-13: Updated PubMed.

Publication List

Imami K, Selbach M, Ishihama Y, Monitoring mitochondrial translation by pulse SILAC. J Biol Chem, 299(2):102865(2023) [pubmed]

Imami K, Selbach M, Ishihama Y, Monitoring mitochondrial translation by pulse SILAC. J Biol Chem, 299(2):102865(2023) [pubmed]

Keyword List

submitter keyword: Mitochondria, translation, ribosome, SILAC

submitter keyword: Mitochondria, translation, ribosome, SILAC

Contact List

Yasushi Ishihama
lab head
Koshi Imami
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001007/