PXD022472

PXD022472 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of Brucella melitensis 16M and REV1
Description	Brucellosis is a zoonosis of the Mediterranean and Middle-East regions linked to important economic losses and reduced animal welfare. To date, no effective diagnostic and/or prophylactic measures are available for the control of brucellosis, due to the residual virulence of the bacterial strain administered for vaccinal purposes and the difficulties in distinguishing vaccinated from infected animals. To overcome these issues, studies are desired to elucidate the bacterial biology and the pathogenetic mechanisms of both the vaccinal strain and the pathogenic strains. Here, we employed a label-free shotgun proteomics to investigate the protein repertoire of the vaccinal strain B. melitensis Rev.1 and compare it with the proteome of the Brucella melitensis 16 M, one of the most common field strains isolated from ruminants. Comparative proteomics profiling underlines common and diverging traits between the two strains, providing suggestions on the potential biochemical routes responsible of the residual virulence of the vaccinal strain; whilst the diverging traits are suggestive biochemical signatures to be further investigated to provide an optimized diagnostics capable of discriminating the vaccinated from infected animals.
HostingRepository	PRIDE
AnnounceDate	2021-05-20
AnnouncementXML	Submission_2021-05-20_04:19:06.433.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022472
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alessio soggiu
SpeciesList	scientific name: Brucella melitensis bv. 1 str. 16M; NCBI TaxID: 224914;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-11 07:36:59	ID requested
⏵ 1	2021-05-20 04:19:07	announced

Tilocca B, Soggiu A, Greco V, Sacchini F, Garofolo G, Paci V, Bonizzi L, Urbani A, Tittarelli M, Roncada P, is a useful toolbox for developing prophylactic interventions in a One-Health context. One Health, 13():100253(2021) [pubmed]

submitter keyword: Brucella melitensis, label-free, 16m, REV1, label-free, HDMSE

Alessio Soggiu
contact affiliation	Department of Biomedical, Surgical and Dental Sciences. University of Milan, Milan, Italy
contact email	alessio.soggiu@unimi.it
lab head
alessio soggiu
contact affiliation	University of Milan
contact email	alessio.soggiu@unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022472
     1. Label: PRIDE project
     2. Name: Comparative proteomics of Brucella melitensis 16M and REV1