PXD022466

PXD022466 is an original dataset announced via ProteomeXchange.

Title	Rat retina and optic nerve LC-MS/MS
Description	Various retinal disorder such as glaucomatous, retinal ischemia reperfusion, and traumatic optic neuropathy, are involved in the pathogenesis of neurodegeneration via glutamate exicitotoxicity. However, the proteomic characteristics and modulation of the neural-microenvironment with NMDA-induced neurodegeneration in retina and optic nerve remain partly understood. We established a protein sketch of NMDA-induced injury by comparing the proteomes of the PBS-operated, NMDA-operated and control groups. We carried out mass spectrometry-based label-free quantitative proteomics to investigate the exicitotoxic neurodegeneration mechanisms and identify key proteins that regulated neural cell death related signaling pathway in retina and optic nerve spatially. Using LC-MS/MS proteomics analysis, in total, we identified 3532 proteins in retina, 2593 proteins in optic nerve. According to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), the protein changes and energy metabolism in retina and optic nerve tissue were comprehensively evaluated.
HostingRepository	PRIDE
AnnounceDate	2021-12-30
AnnouncementXML	Submission_2021-12-30_14:34:28.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingge Suo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-11 02:37:56	ID requested
⏵ 1	2021-12-30 14:34:29	announced

Suo L, Dai W, Chen X, Qin X, Li G, Song S, Zhang D, Zhang C, Proteomics analysis of N-methyl-d-aspartate-induced cell death in retinal and optic nerves. J Proteomics, 252():104427(2022) [pubmed]

submitter keyword: Rat, retina, optic nerve, LC-MS/MS

Chun Zhang
contact affiliation	Department of Ophthalmology, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China
contact email	zhangc1@yahoo.com
lab head
Lingge Suo
contact affiliation	Peking University Third Hospital
contact email	suolingge_1019@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD022466

PRIDE project URI

• PRIDE
  1. PXD022466
     1. Label: PRIDE project
     2. Name: Rat retina and optic nerve LC-MS/MS