PXD022460

PXD022460 is an original dataset announced via ProteomeXchange.

Title	Functional proteomics of membrane lipid domains after Brij 98 solubilization of uninduced and phenobarbital-induced rat liver microsomes: Defining the membrane localization of the P450 enzyme system
Description	In this study, we use Brij 98 to determine the lipid domain distribution of the ER proteome in rat liver microsomes with emphasis on that of the P450 enzyme system. Because of the profound effects of phenobarbital treatment on protein expression and lipid biosynthesis, we will examine the liver ER proteomes from both uninduced and phenobarbital-treated rats in order to determine if protein and lipid variability influence the domain localization of proteins. To ascertain possible functional roles associated with membrane domain localization, we will apply G.O. analysis and Ingenuity Pathway Analysis (IPA) to the proteins from the identified membrane domains. The biological processes, molecular functions, and cellular pathways associated with the membrane lipid domains will be described, and the potential biological implications of these associations will be discussed.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_18:38:35.491.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD022460
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessie Guidry
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-11 02:37:21	ID requested
⏵ 1	2026-04-26 18:38:36	announced

10.1124/dmd.121.000752;

10.6019/PXD022460;

Reed JR, Guidry JJ, Backes WL, Proteomic and Bioinformatics Analysis of Membrane Lipid Domains after Brij 98 Solubilization of Uninduced and Phenobarbital-Induced Rat Liver Microsomes: Defining the Membrane Localization of the P450 Enzyme System. Drug Metab Dispos, 50(4):374-385(2022) [pubmed]

submitter keyword: TMT discovery based proteomics, p450s,liver microsomes

Jessie J Guidry
contact affiliation	Department of Biochemistry and Molecular Biology Proteomics Core Facility LSUHSC New Orleans,LA 70112
contact email	jjguid@lsuhsc.edu
lab head
Jessie Guidry
contact affiliation	Louisiana State University Health Sciences Center Department of Biochemistry and Molecular Biology LSUHSC Proteomics Core Facility
contact email	jjguid@lsuhsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022460
     1. Label: PRIDE project
     2. Name: Functional proteomics of membrane lipid domains after Brij 98 solubilization of uninduced and phenobarbital-induced rat liver microsomes: Defining the membrane localization of the P450 enzyme system