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PXD022456-2

PXD022456 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConfirmation of plant viral proteins and identification of specific viral strains by nanoLC-ESI-Q-TOF using single-leaf-tissue samples
DescriptionAn efficient protein extraction protocol without any precipitation steps was applied to plant samples from ten economically important plant hosts. Viral proteins from fourteen important viruses (WDV, BYDV/BYDV-PAV, BMV, TAV, CaMV, TMV, TVCV, PPV, TuMV, BCMV, SrMV, SCMV, TRSV, BBWV-2) from seven different families (Geminiviridae, Luteoviridae, Bromoviridae, Caulimoviridae, Virgaviridae, Potyviridae, Secoviridae) were detected in infected samples. Protein database of host proteins and potential pathogen proteins was assembled separately for each host and based on existing online plant virus pathogen lists. For PPV, WDV, BYDV a succesfull discrimination to virus strains (as demonstrated for PPV, WDV) or distinct disease species (BYDV) was also demonstrated.
HostingRepositoryPRIDE
AnnounceDate2020-11-20
AnnouncementXMLSubmission_2020-11-25_02:40:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPavel Cejnar
SpeciesList scientific name: Phaseolus vulgaris; NCBI TaxID: 3885; scientific name: Prunus armeniaca; NCBI TaxID: 36596; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Chenopodium giganteum; NCBI TaxID: 434242; scientific name: Sorghum bicolor; NCBI TaxID: 4558; scientific name: Prunus domestica; NCBI TaxID: 3758; scientific name: Zea mays (Maize); NCBI TaxID: 4577; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513; scientific name: Brassica rapa subsp. pekinensis; NCBI TaxID: 51351; scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationListiodoacetamide derivatized residue
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-10 07:56:25ID requested
12020-11-20 07:02:47announced
22020-11-25 02:40:52announced2020-11-25: Updated publication reference for PubMed record(s): 33228257.
Publication List
Cejnar P, Ku, č, kov, á Š, Š, antr, ů, č, ek J, Glasa M, Kom, í, nek P, Mih, á, lik D, Slav, í, kov, á L, Lei, š, ov, á, -Svobodov, á L, Smirnova T, Hynek R, Kundu JK, Ry, š, á, nek P, Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples. Pathogens, 9(11):(2020) [pubmed]
Keyword List
submitter keyword: plant, viral proteins, LC-MSMS, WDV, BYDV/BYDV-PAV, BMV, TAV, CaMV, TMV, TVCV, PPV, TuMV, BCMV, SrMV, SCMV, TRSV, BBWV-2
Contact List
Pavel Cejnar
contact affiliationDepartment of Computing and Control Engineering, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha 6, Czech Republic
contact emailpavel.cejnar@vscht.cz
lab head
Pavel Cejnar
contact affiliationDepartment of Computing and Control Engineering, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha 6, Czech Republic
contact emailpavel.cejnar@vscht.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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