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PXD022454-1

PXD022454 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
DescriptionHistone post-translational modifications (hPTMs) belong to decisive epigenetic marks affecting, among others, cell cycle and protein interactions. hPTMs have been studied by both antibody- and mass spectrometry-based methods for years, however, those analyses are still challenging, mainly due to multiple histone variants and their modification diversity arising from high content of reactive amine groups in the amino acid sequence. Here, we introduce trimethylacetic anhydride (TMA) as a new reagent for efficient histone alkylation, a necessary approach prior to hPTMs analysis using bottom-up proteomics. TMA reagent is applied for derivatization of unmodified amine groups at lysine residues as well as for subsequent alkylation of newly generated amine groups at peptide N-termini after trypsin digestion. Derivatization reaction is facilitated by microwave irradiation, which also reduces incubation time to minute intervals. We demonstrate that histone alkylation with TMA represents a reliable technique with high yield of fully derivatized peptides, hence an effective alternative to conventionally used methods. More than 98 % and 99 % labelling efficiency of TMA was achieved for histone H4 and H3, respectively, ensuring an accurate quantification of peptide forms. Trimethylacetylation substantially improves chromatographic separation of peptide forms which is a prerequisite for direct quantification based on signal extracted from MS1 data. For this purpose, software tools widely adopted within proteomics community can be used without a need of additional computational advances. Based on a thorough side-by-side comparison of TMA performance with well-adopted propionylation we comment on the impact of histone derivatization on the hPTMs monitoring in biological samples.
HostingRepositoryPanoramaPublic
AnnounceDate2021-06-09
AnnouncementXMLSubmission_2021-06-09_11:52:23.214.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterHana Kucharikova
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListAcetyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-10 06:29:33ID requested
12021-06-09 11:52:23announced
22021-06-15 06:31:36announced: Added publication
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: o Histone derivatization, trimethylacetic anhydride, epigenetics, mass spectrometry
Contact List
Zbynek Zdrahal
contact affiliation1 Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic 2 Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
Hana Kucharikova
contact affiliationCEITEC MU, Faculty of Science MU, Czech republic
contact emailhana.kucharikova@ceitec.muni.cz
dataset submitter
Full Dataset Link List
Panorama Public dataset URI