PXD022452

PXD022452 is an original dataset announced via ProteomeXchange.

Title	Molecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells
Description	In poultry, in vitro derived primordial germ cells (PGCs) represent an important tool for management of genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs through in vitro steps, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal chicken male (ZZ) and female (ZW) PGCs expanded in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. The proteins found to be differentially abundant in chicken male and female PGCs indicated their early sexual identity. Many of the proteins up-accumulated in male PGCs were encoded by genes strongly enriched in the sexual chromosome Z. This suggests that the known lack of dosage compensation of the transcription of Z-linked genes between sexes persists at protein level in PGCs, and that this may be a key factor of their autonomous sex differentiation. Male and female PGCs up-accumulated protein sets were associated with differential biological processes, and contained proteins biologically relevant for male and female germ cell development respectively. This study presents first evidence on early predetermined sex specific cell fate of chicken PGCs that will help to understand their sexual physiological specificities and enable more precise sex-specific adaptation of in vitro culture conditions.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_08:06:06.897.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022452
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Valerie LABAS
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-10 04:02:48	ID requested
⏵ 1	2021-11-03 08:06:08	announced

Soler L, Alves S, Brionne A, Jacques A, Gu, é, rin V, Cherif-Feildel M, Combes-Soia L, Fouch, é, court S, Th, é, lie A, Blesbois E, McGrew MJ, Labas V, Govoroun MS, Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages. Sci Rep, 11(1):19236(2021) [pubmed]

submitter keyword: Chicken, primordial germ cells, sex differentiation

Marina Govoroun
contact affiliation	INRAE Val de Loire France
contact email	marina.govoroun@inrae.fr
lab head
Valerie LABAS
contact affiliation	INRAE
contact email	valerie.labas@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022452
     1. Label: PRIDE project
     2. Name: Molecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells