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PXD022452

PXD022452 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells
DescriptionIn poultry, in vitro derived primordial germ cells (PGCs) represent an important tool for management of genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs through in vitro steps, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal chicken male (ZZ) and female (ZW) PGCs expanded in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. The proteins found to be differentially abundant in chicken male and female PGCs indicated their early sexual identity. Many of the proteins up-accumulated in male PGCs were encoded by genes strongly enriched in the sexual chromosome Z. This suggests that the known lack of dosage compensation of the transcription of Z-linked genes between sexes persists at protein level in PGCs, and that this may be a key factor of their autonomous sex differentiation. Male and female PGCs up-accumulated protein sets were associated with differential biological processes, and contained proteins biologically relevant for male and female germ cell development respectively. This study presents first evidence on early predetermined sex specific cell fate of chicken PGCs that will help to understand their sexual physiological specificities and enable more precise sex-specific adaptation of in vitro culture conditions.
HostingRepositoryPRIDE
AnnounceDate2021-11-03
AnnouncementXMLSubmission_2021-11-03_08:06:06.897.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD022452
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterValerie LABAS
SpeciesList scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-10 04:02:48ID requested
12021-11-03 08:06:08announced
Publication List
Soler L, Alves S, Brionne A, Jacques A, Gu, é, rin V, Cherif-Feildel M, Combes-Soia L, Fouch, é, court S, Th, é, lie A, Blesbois E, McGrew MJ, Labas V, Govoroun MS, Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages. Sci Rep, 11(1):19236(2021) [pubmed]
Keyword List
submitter keyword: Chicken, primordial germ cells, sex differentiation
Contact List
Marina Govoroun
contact affiliationINRAE Val de Loire France
contact emailmarina.govoroun@inrae.fr
lab head
Valerie LABAS
contact affiliationINRAE
contact emailvalerie.labas@inrae.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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