PXD022452 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells |
Description | In poultry, in vitro derived primordial germ cells (PGCs) represent an important tool for management of genetic resources. However, several studies have highlighted sexual differences exhibited by PGCs through in vitro steps, which may compromise their reproductive capacities. To understand this phenomenon, we compared the proteome of pregonadal chicken male (ZZ) and female (ZW) PGCs expanded in vitro by quantitative proteomic analysis using a GeLC-MS/MS strategy. The proteins found to be differentially abundant in chicken male and female PGCs indicated their early sexual identity. Many of the proteins up-accumulated in male PGCs were encoded by genes strongly enriched in the sexual chromosome Z. This suggests that the known lack of dosage compensation of the transcription of Z-linked genes between sexes persists at protein level in PGCs, and that this may be a key factor of their autonomous sex differentiation. Male and female PGCs up-accumulated protein sets were associated with differential biological processes, and contained proteins biologically relevant for male and female germ cell development respectively. This study presents first evidence on early predetermined sex specific cell fate of chicken PGCs that will help to understand their sexual physiological specificities and enable more precise sex-specific adaptation of in vitro culture conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-03 |
AnnouncementXML | Submission_2021-11-03_08:06:06.897.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022452 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Valerie LABAS |
SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-10 04:02:48 | ID requested | |
⏵ 1 | 2021-11-03 08:06:08 | announced | |
Publication List
Soler L, Alves S, Brionne A, Jacques A, Gu, é, rin V, Cherif-Feildel M, Combes-Soia L, Fouch, é, court S, Th, é, lie A, Blesbois E, McGrew MJ, Labas V, Govoroun MS, Protein expression reveals a molecular sexual identity of avian primordial germ cells at pre-gonadal stages. Sci Rep, 11(1):19236(2021) [pubmed] |
Keyword List
submitter keyword: Chicken, primordial germ cells, sex differentiation |
Contact List
Marina Govoroun |
contact affiliation | INRAE Val de Loire France |
contact email | marina.govoroun@inrae.fr |
lab head | |
Valerie LABAS |
contact affiliation | INRAE |
contact email | valerie.labas@inrae.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022452
- Label: PRIDE project
- Name: Molecular sexual identity of chicken in vitro derived pre-gonadal primordial germ cells