PXD022440

PXD022440 is an original dataset announced via ProteomeXchange.

Title	Identification of Plk1 interacting proteins by XChIP-MS
Description	Polo-like kinase 1 (Plk1) is an important protein kinase for checkpoint recovery and adaptation in response to DNA damage and replication stress. However, although Plk1 is present in S phase, little is known about its localization and function during unperturbed DNA replication. Using Xenopus laevis egg extracts, mimicking early embryonic replication, we developed a Plk1 chromatin immunoprecipitation approach followed by a proteomic shotgun analysis (XChIP-MS) to identify Plk1 interactions with chromatin and with specific nuclear proteins. We purified and cross-linked nuclei after pre-RC assembly before the start of DNA replication, or later during DNA replication. The DNA was fractionated by sonication and immunoprecipited using anti Plk1 or control antibodies then proteins were analyzed by nanoLC/MS/MS.
HostingRepository	PRIDE
AnnounceDate	2021-08-25
AnnouncementXML	Submission_2021-08-25_06:49:14.075.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022440
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Cornu
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 4600

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-10 01:59:21	ID requested
⏵ 1	2021-08-25 06:49:14	announced

Dataset with its publication pending

submitter keyword: Xenopus laevis, LC-MSMS

David Cornu
contact affiliation	Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France
contact email	david.cornu@i2bc.paris-saclay.fr
lab head
David Cornu
contact affiliation	SICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact email	david.cornu@i2bc.paris-saclay.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022440
     1. Label: PRIDE project
     2. Name: Identification of Plk1 interacting proteins by XChIP-MS