PXD022428

PXD022428 is an original dataset announced via ProteomeXchange.

Title	Supercooled, subzero, high salinity incubations of Cp34H reveal long-term mitigation strategies and detectable (peptide) biomarkers for life on other icy worlds
Description	Colwellia psychrerythraea is a marine psychrophilic bacterium known for its remarkable ability to maintain activity during long-term exposure to extreme subzero temperatures and correspondingly high salinities in sea ice. These microorganisms must have simultaneous adaptations to high salinity and low temperature to survive, be metabolically active, or grow in the ice. Here we report results obtained through an experimental design that allowed us to monitor culturability, activity, and proteomic signatures of Colwellia psychrerythraea strain 34H (Cp34H) to subzero temperature, salinity, and nutrient availability by performing long-term incubations in eight different conditions. Shotgun proteomics revealed novel metabolic strategies used to maintain culturability in response to each independent experimental variable, particularly in pathways regulating carbon, nitrogen, and fatty acid metabolism. For the first time, statistical analysis of differential abundances of proteins uniquely identified in these isolated conditions provide metabolism-specific protein biosignatures indicative of growth or survival in either increased salinity, decreased temperature, or nutrient limitation. Additionally, to aid in the search for extant life on other icy worlds, analysis of detected short peptides enriched and retained in -10oC incubations for four months identified over 400 potential biosignatures that could indicate the presence of terrestrial-like cold-active or halophilic metabolisms on other icy worlds.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:48:19.768.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miranda Mudge
SpeciesList	scientific name: Colwellia psychrerythraea 34H; NCBI TaxID: 167879;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-09 03:24:32	ID requested
⏵ 1	2022-02-16 09:48:20	announced

Mudge MC, Nunn BL, Firth E, Ewert M, Hales K, Fondrie WE, Noble WS, Toner J, Light B, Junge KA, Subzero, saline incubations of Colwellia psychrerythraea reveal strategies and biomarkers for sustained life in extreme icy environments. Environ Microbiol, 23(7):3840-3866(2021) [pubmed]

submitter keyword: biomarkers, culturability, life detection, Cp34H, Arctic, sea-ice, subzero, psychrophile, proteomics

Brook Nunn
contact affiliation	Nunn Lab, University of Washington, Department of Genome Sciences, Seattle, WA USA
contact email	brookh@uw.edu
lab head
Miranda Mudge
contact affiliation	Department of Molecular and Cellular Biology, University of Washington
contact email	mudge832@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022428
     1. Label: PRIDE project
     2. Name: Supercooled, subzero, high salinity incubations of Cp34H reveal long-term mitigation strategies and detectable (peptide) biomarkers for life on other icy worlds