PXD022424

PXD022424 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling of arginine methylation mediated by PRMT family reveals a critical role of arginine methylation in RNA splicing and cancer cell growth
Description	Protein arginine methylation, catalyzed by the protein arginine methyltransferase (PRMT) family, is recognized as a widespread post-translational modification (PTM) with implications in a plethora of biological processes in eukaryotes. PRMT proteins were classified into three types, type I, II and III, according to the final methyl-arginine products generated. Despite that thousands of substrates have been identified for Type I and II PRMTs, a full scope of arginine methylation catalyzed by the only type III PRMT, PRMT7, as well as its connection with that of type I and II PRMTs remain unknown, limiting our understanding of the network of arginine methylation and its functions in cells. In this study, global profiling of PRMT4 (type I), PRMT5 (type II) and PRMT7 (type III) substrates (also referred as methylome) revealed that PRMT7 methylated a GAR (glycine and arginine) motif similar as PRMT5, while PRMT4 uniquely methylated a motif in which proline was highly enriched. PRMT4, 5 and 7-methylome were all enriched with proteins functioning in mRNA splicing.
HostingRepository	PRIDE
AnnounceDate	2021-01-27
AnnouncementXML	Submission_2021-04-22_22:31:26.684.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wen Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-09 03:24:11	ID requested
1	2021-01-26 23:15:20	announced
⏵ 2	2021-04-22 22:31:27	announced	2021-04-23: Updated publication reference for PubMed record(s): 33782401.

Li WJ, He YH, Yang JJ, Hu GS, Lin YA, Ran T, Peng BL, Xie BL, Huang MF, Gao X, Huang HH, Zhu HH, Ye F, Liu W, Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth. Nat Commun, 12(1):1946(2021) [pubmed]

submitter keyword: PRMTs,arginine methylation,RNA splicing

Wen Liu
contact affiliation	School of Pharmaceutical Sciences,Xiamen University
contact email	W2liu@xmu.edu.cn
lab head
Wen Liu
contact affiliation	Xiamen University
contact email	W2liu@xmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022424
     1. Label: PRIDE project
     2. Name: Proteomics profiling of arginine methylation mediated by PRMT family reveals a critical role of arginine methylation in RNA splicing and cancer cell growth