PXD022413

PXD022413 is an original dataset announced via ProteomeXchange.

Title	An early endosomal network participates to enterocyte functions and intestinal homeostasis
Description	Membrane trafficking is defined as the vesicular transport of molecules throughout the cell. In intestinal enterocytes, defects in endocytic/recycling pathways impair their function and are linked to genetic diseases. How does trafficking regulate intestinal tissue homeostasis is poorly understood. Using the Drosophila intestine as an in vivo model system, we investigated enterocyte-specific functions for early endosomal trafficking in gut homeostasis. We focused on the small GTPase Rab21 that regulates specific early endosomal trafficking steps. Rab21-depleted guts showed severe intestinal morphology abnormalities, with deregulated homeostasis associated with a gain in mitotic cells and increased cell death. Increased in both apoptosis and Yki signaling were responsible for compensatory proliferation and tissue inflammation. Using a RNAi screen, we identified autophagy and specific membrane trafficking regulators phenocopying Rab21 loss. We further showed that Rab21-induced hyperplasia was rescued by inhibition of Egfr signaling, and we identified improperly trafficked cargoes in enterocytes depleted of Rab21. Our data shed light on an important role for the early endosomal protein Rab21, and early endosomal trafficking in enterocytes-mediated intestinal homeostasis.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_21:42:13.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steve Jean
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-06 08:30:55	ID requested
⏵ 1	2022-08-11 21:42:22	announced

Nassari S, Lacarri, è, re-Ke, ï, ta C, L, é, vesque D, Boisvert FM, Jean S, Rab21 in enterocytes participates in intestinal epithelium maintenance. Mol Biol Cell, 33(4):ar32(2022) [pubmed]

submitter keyword: Drosophila, intestine, Rab21, TMT

Steve Jean
contact affiliation	Faculté de Médecine et des Sciences de la Santé Department of Immunology and Cell Biology Université de Sherbrooke
contact email	steve.jean@usherbrooke.ca
lab head
Steve Jean
contact affiliation	Université de Sherbrooke
contact email	steve.jean@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022413
     1. Label: PRIDE project
     2. Name: An early endosomal network participates to enterocyte functions and intestinal homeostasis