PXD022406

PXD022406 is an original dataset announced via ProteomeXchange.

Title	Cross-species proteomic comparison of whole cell lysates, outer membrane vesicles and membrane fractions of Francisella tularensis subsp. tularensis versus subsp. holarctica
Description	Secretion of outer membrane vesicles (OMV) presents an important phenomenon in Gram-negative bacteria and they play multiple roles in their lifestyle including virulence and host-pathogen interaction. Francisella tularensis secretes unusually shaped tubular OMV. We here present a proteomic comparison of whole cell lysates, OMV and membrane fractions derived from two F. tularensis strains: a moderately virulent subsp. holarctica strain FSC200 and highly virulent subsp. tularensis SchuS4 strain. We describe a novel approach to the cross-species proteomic comparison based on creating an intersection protein database from the individual single-species databases. This approach proved to be less prone to quantification errors arising from differences in the protein sequences than using the individual databases. The consecutive comparison of the subproteomes of OMV and membranes of the two strains allowed us to distinguish the differences in protein amounts caused by global expression changes from those caused by preferential protein packing to OMV or membrane. Amongst the most different proteins we detected proteins involved in the biosynthesis and metabolism of the bacterial envelope components like O-antigen, lipid A, phospholipids, and fatty acids, as well as some of the major structural outer membrane proteins.
HostingRepository	PRIDE
AnnounceDate	2021-02-08
AnnouncementXML	Submission_2021-02-07_23:06:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Klimentova
SpeciesList	scientific name: Francisella tularensis subsp. holarctica FSC200; NCBI TaxID: 351581; scientific name: Francisella tularensis; NCBI TaxID: 263; scientific name: Francisella tularensis subsp. tularensis SCHU S4; NCBI TaxID: 177416;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-06 07:03:49	ID requested
⏵ 1	2021-02-07 23:06:08	announced

Klimentova J, Rehulka P, Pavkova I, Kubelkova K, Bavlovic J, Stulik J, . J Proteome Res, 20(3):1716-1732(2021) [pubmed]

submitter keyword: Francisella tularensis, SchuS4, FSC200, outer membrane vesicles, cross-species proteomic comparison, Q-Exactive

Jiri Stulik
contact affiliation	Department of Molecular Pathology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Jana Klimentova
contact affiliation	Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jana.klimentova@unob.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022406
     1. Label: PRIDE project
     2. Name: Cross-species proteomic comparison of whole cell lysates, outer membrane vesicles and membrane fractions of Francisella tularensis subsp. tularensis versus subsp. holarctica