PXD022396

PXD022396 is an original dataset announced via ProteomeXchange.

Title	Shulin packages axonemal outer dynein arms for ciliary targeting
Description	The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During cilio-genesis, these ∼1.8 MDa complexes are assembled in the cytoplasm and targeted to cilia via an unknown mechanism. Here we use the ciliate Tetrahymena to identify two novel factors (Q22YU3 and Q22MS1) which bind ODAs in the cytoplasm and are required for their delivery to cilia. We show that Q22YU3, which we name Shulin, locks the ODA motor domains into a closed conformation and inhibits motor activity. Cryo-EM reveals how Shulin stabilizes this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_14:23:51.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Skehel
SpeciesList	scientific name: Tetrahymena thermophila CU428; NCBI TaxID: 452467;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-06 01:38:47	ID requested
⏵ 1	2021-09-09 14:23:51	announced

Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP, Shulin packages axonemal outer dynein arms for ciliary targeting. Science, 371(6532):910-916(2021) [pubmed]

submitter keyword: Tetrahymena thermophila, lc-ms/ms, Cilia, dynein, Cryo-EM, flagella

Andrew P Carter
contact affiliation	Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
contact email	cartera@mrc-lmb.cam.ac.uk
lab head
Mark Skehel
contact affiliation	MRC LMB
contact email	mskehel@mrc-lmb.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022396
     1. Label: PRIDE project
     2. Name: Shulin packages axonemal outer dynein arms for ciliary targeting