PXD022393 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Circadian Regulation of Protein Cargo in Exosomes |
Description | The circadian clock controls many aspects of physiology, but it remains undescribed whether extracellular vesicles (including exosomes) involved in cell-cell communications between tissues are regulated in a circadian pattern. We demonstrate a 24 h rhythmic abundance of individual proteins in exosomes using liquid chromatography-mass spectrometry in circadian-synchronised tendon fibroblasts. Further, the release of exosomes enriched in RNA-binding proteins was temporally separated from those enriched in cytoskeletal and matrix proteins, which peaked during the end of the light phase. Finally, we targeted the protein sorting mechanism in the exosome biogenesis pathway and established (by knockdown of circadian-regulated flotillin-1) that matrix metalloproteinase 14 abundance in tendon fibroblast exosomes is under flotillin-1 regulation. In conclusion, we have identified proteomic time signatures for exosomes released by tendon fibroblasts which supports the view that the circadian clock regulates protein cargo in exosomes involved in cell-cell crosstalk. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_13:59:32.982.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Erwin Schoof |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-06 01:38:29 | ID requested | |
⏵ 1 | 2022-05-19 13:59:34 | announced | |
Publication List
Yeung CC, Dondelinger F, Schoof EM, Georg B, Lu Y, Zheng Z, Zhang J, Hannibal J, Fahrenkrug J, Kjaer M, Circadian regulation of protein cargo in extracellular vesicles. Sci Adv, 8(14):eabc9061(2022) [pubmed] |
Keyword List
submitter keyword: LC-MS, exosomes, circadian rhythm |
Contact List
Michael Kjaer |
contact affiliation | Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, and Center for Healthy Aging, University of Copenhagen, Copenhagen, Denmark. |
contact email | michaelkjaer@sund.ku.dk |
lab head | |
Erwin Schoof |
contact affiliation | Technical University of Denmark |
contact email | erws@dtu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD022393
- Label: PRIDE project
- Name: Circadian Regulation of Protein Cargo in Exosomes