PXD022383

PXD022383 is an original dataset announced via ProteomeXchange.

Title	ULK1 regulated phosphatases in mouse and human cells
Description	In the project “ULK1 regulated phosphatases” by Zehan Hu, Devanarayanan Siva Sankar, Björn Stork and Jörn Dengjel two sets of bottom-up MS-based chemical proteomics experiments were performed in which phosphatase complexes were enriched based on their activity using microcystin-LR coupled sepharose beads. (1) Three biological replicates of MEFs (triple labeling) were performed comparing phosphatase activity (36 raw files labeled "2018"). ULK1 MEFs were compared DKO MEFs, both in rapamycin conditions. Empty beads were used as negative control (Ctrl). Following experiments were performed: (2) Three biological replicates of A549 cells (triple labeling) were performed comparing phosphatase activity (30 raw files labeled "A549"). A549 cells in fed conditions (DMEM) were compared to rapamycin treated cells (Rapa). Empty beads were used as negative control (Ctrl).
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_04:53:56.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joern Dengjel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 06:09:52	ID requested
⏵ 1	2021-11-03 04:53:57	announced

Hu Z, Sankar DS, Vu B, Leytens A, Vionnet C, Wu W, Stumpe M, Mart, í, nez-Mart, í, nez E, Stork B, Dengjel J, ULK1 phosphorylation of striatin activates protein phosphatase 2A and autophagy. Cell Rep, 36(13):109762(2021) [pubmed]

submitter keyword: autophagy, MEFs, A549, chemical proteomics, SILAC

Joern Dengjel
contact affiliation	Department of Biology, University of Fribourg Chemin du Musée 10, 1700 Fribourg, Switzerland T +41 26 300 8631 E joern.dengjel@unifr.ch
contact email	joern.dengjel@unifr.ch
lab head
Joern Dengjel
contact affiliation	University of Fribourg
contact email	joern.dengjel@medizin.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD022383

PRIDE project URI

• PRIDE
  1. PXD022383
     1. Label: PRIDE project
     2. Name: ULK1 regulated phosphatases in mouse and human cells