PXD022381

PXD022381 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomic and metabolomic profiling of Phytophthora cinnamomi attack on sweet chestnut (Castanea sativa) reveals distinct molecular reprogramming proximal to the infection site and away from it
Description	Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora-woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA) indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity
HostingRepository	PRIDE
AnnounceDate	2020-11-26
AnnouncementXML	Submission_2020-11-26_08:13:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Castanea sativa; NCBI TaxID: 21020;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 06:09:49	ID requested
⏵ 1	2020-11-26 08:13:03	announced

Saiz-Fern, á, ndez I, Milenkovi, ć I, Berka M, Č, ern, ý M, Tom, š, ovsk, ý M, Brzobohat, ý B, Kerchev P, ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It. Int J Mol Sci, 21(22):(2020) [pubmed]

submitter keyword: sweet chestnut

Phytophthora cinnamomi

proteomics

Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD022381

PRIDE project URI

• PRIDE
  1. PXD022381
     1. Label: PRIDE project
     2. Name: Integrated proteomic and metabolomic profiling of Phytophthora cinnamomi attack on sweet chestnut (Castanea sativa) reveals distinct molecular reprogramming proximal to the infection site and away from it