PXD022380

PXD022380 is an original dataset announced via ProteomeXchange.

Title	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
Description	Exposures to low doses of ionizing radiation are relevant since most environmental, diagnostic radiology and occupational exposures lie in this region. However, the molecular mechanisms that drive cellular responses at these doses, and the subsequent health outcomes, remain unclear. A local monazite-rich high level natural radiation area (HLNRA) in the state of Kerala on the south-west coast of Indian subcontinent show radiation doses extending from ≤1 to ≥45 mGy/y and thus, serve as a model resource to understand low dose mechanisms directly on healthy humans. We performed quantitative discovery proteomics based on multiplexed isobaric tags (iTRAQ) coupled with LC-MS/MS on human peripheral blood mononuclear cells from HLNRA individuals. Several proteins involved in diverse biological processes such as DNA repair, RNA processing, chromatin modifications and cytoskeletal organization showed distinct expression in HLNRA individuals, suggestive of both recovery and adaptation to low dose radiation. In protein-protein interaction (PPI) networks, YWHAZ (14-3-3ζ) emerged as the top-most hub protein that may direct phosphorylation driven pro-survival cellular processes against radiation stress. PPI networks also identified an integral role for the cytoskeletal protein ACTB, signaling protein PRKACA; and the molecular chaperone HSPA8. The data will allow better integration of radiation biology and epidemiology for risk assessment.
HostingRepository	PRIDE
AnnounceDate	2021-01-28
AnnouncementXML	Submission_2021-01-28_02:31:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anu Ghosh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 02:23:33	ID requested
⏵ 1	2021-01-28 02:31:58	announced

Nishad S, Chauhan PK, Sowdhamini R, Ghosh A, Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins. Sci Rep, 11(1):1777(2021) [pubmed]

submitter keyword: High level natural radiation area (HLNRA), Chronic low dose radiation, Dose response analysis, iTRAQ, Stress response

Anu Ghosh
contact affiliation	Radiation Signaling Group, Radiation Biology & Health Sciences Division, Bio-Science Group, Bhabha Atomic Research Centre (BARC), Mumbai - 400 085, India and Homi Bhabha National Institute (HBNI), Mumbai - 400 094, India
contact email	anugh@barc.gov.in
lab head
Anu Ghosh
contact affiliation	Bhabha Atomic Reasearch Centre
contact email	anugh@barc.gov.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022380

PRIDE project URI

• PRIDE
  1. PXD022380
     1. Label: PRIDE project
     2. Name: Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins