PXD022378

PXD022378 is an original dataset announced via ProteomeXchange.

Title	Subcellular proteomics of murine ESC-derived neurons
Description	We studied the effects of different stimuli on on the subcellular proteome of neurons differentiated from murine embryonic stem cells (ESC). We focused on the changes in the chromatin-bound fraction to elucidate specific differences in the gene regulatory machinery activated upon stimulation with Brain-derived neurotrophic factor (BDNF) or a general membrane-depolarization stimulus, potassium chloride (KCl).
HostingRepository	PRIDE
AnnounceDate	2022-09-15
AnnouncementXML	Submission_2022-09-15_04:33:43.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik Hammarén
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 02:03:50	ID requested
⏵ 1	2022-09-15 04:33:45	announced

Ibarra IL, Ratnu VS, Gordillo L, Hwang IY, Mariani L, Weinand K, Hammar, é, n HM, Heck J, Bulyk ML, Savitski MM, Zaugg JB, Noh KM, Comparative chromatin accessibility upon BDNF stimulation delineates neuronal regulatory elements. Mol Syst Biol, 18(8):e10473(2022) [pubmed]

submitter keyword: subcellular fractionation, BDNF, KCL, neurons, stimulation, TMT, Thermo Q Exactive plus

Mikhail M Savitski
contact affiliation	Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact email	mikhail.savitski@embl.de
lab head
Henrik Hammarén
contact affiliation	Genome Biology Unit and Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact email	henrik.hammaren@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD022378

PRIDE project URI

• PRIDE
  1. PXD022378
     1. Label: PRIDE project
     2. Name: Subcellular proteomics of murine ESC-derived neurons